WebApr 11, 2024 · Single-nuclei suspension was loaded on a Chromium controller to obtain single-cell GEMS (Gel Beads-In-Emulsions) for the reaction. The snRNA-seq library was prepared with Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics). The snATAC-seq library was prepared with Chromium Next GEM Single Cell ATAC Library & Gel … WebChromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore … Multiomic profiling of immune diversity, cell by cell Chromium Single Cell Immune …
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WebAug 2, 2024 · Single cells were isolated from patient-derived breast using Tumor Dissociation Kit protocol (Miltenyi Biotec, 130-095-929). Single cells were processed using the Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1 and Chromium Next GEM Chip G Single Cell Kit according to the manufacturer’s instructions in the … WebChromium Next GEM Chip G Single Cell Kit1, 48 rxns 1000120 Chromium Next GEM Chip G Single Cell Kit1, 16 rxns 1000127 Chromium Next GEM Single Cell 3’ Library Construction Kit v3.11, 16 rxns 1000157 Chromium i7 Multiplex Kit, 96 rxns 120262 Chromium Controller & Next GEM Accessory Kit1, 12 Mo. Warranty 1000202 … WebApr 11, 2024 · These were sorted and permeabilized, then washed and counted for use in subsequent steps. The 10× Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression library preparation kit was implemented (Product Code 1000285). Libraries were loaded and run on a Nextseq 500. 5.4. Single cell multiomics analysis … rayco storage lifts