Jeg-3 cell line

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August undefined, 2024

Web12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC … Webseed out at ca. 1 x 10 6 cells/80 cm 2; maintain at ca. 10 x 10 6 cells/175 cm 2; split confluent culture up to 1:10 every 3-4 days using trypsin/EDTA. Incubation: at 37 °C with …

HTR-8/SVneo cell line contains a mixed population of cells

Web4 mar 2024 · In this system, the trophoblast-derived JEG-3 cell line is co-cultured with human brain microvascular endothelial cells attached to microcarrier beads in a rotating bioreactor. Here, we report that 3D culture of JEG-3/HBMEC spheroids promote JEG-3 cells differentiation revealed by the formation of syncytia and production of β human … Web13 mar 2024 · The culture of JEG-3 cells (a human choriocarcinoma cell line) has been described previously . To study the cytotoxic effect of α-solanine on JEG-3 cells, cells were cultured in 96-well plates overnight, followed by treatment with different concentrations of α-solanine (10, 20, 30, 40 and 50 μM) or vehicle (0.5% v/v DMSO) for 24 h. rainbow gateway cloncurry contact

Embedding similarities between embryos and circulating tumor cells …

Web15 mar 2024 · After electroporation and drug selection, a successful HLA-G knockout JEG-3 cell line (JEG-3 KO cells) was established, and its efficacy was first validated by PCR and western blotting . Additional functional validation was performed on the well-established functions of HLA-G, including the inhibition of natural killer cell cytotoxicity and the … Web1 dic 2024 · The aim was to evaluate changes in the NK cell phenotype and function in the presence of IL-15, IL-18 and JEG-3, a trophoblast cell line. When cocultured with JEG-3 … Web30 ott 2024 · The most commonly used cell line in studies on human placental transport is the choriocarcinoma cell line BeWo, ... The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells. Toxicol. Lett. 2013, 218, 200–206. [Google Scholar] Chen, Z.S ... rainbow gateway cloncurry

Human choriocarcinoma cell line JEG-3 produces and secretes …

Leibniz Institute JEG-3 DSMZ

Web4 mar 2016 · We used the RWV bioreactor to develop a 3D-based cell coculture model that induces high levels of spontaneous fusion of a trophoblast cell line, JEG-3 cells. JEG-3 cells cultured in this system exhibit high rates of syncytia formation, which correlates to increased brush border formation and secretion of placental hormones. WebThe cytotoxicity of eight perfluorinated chemicals (PFCs), namely, perfluorobutanoic acid (PFBA), perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorododecanoic acid (PFDoA), perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHxS) and perfluorooctanesulfonate (PFOS) was assessed … rainbow gates houston txWeb13 apr 2024 · To verify the function of miR-526b-5p on the trophoblast cells and explore the intrinsic molecular mechanism, transcriptome sequencing was performed on the HTR8 … rainbow gateway hughenden

"Web"1A6 Cell;1A6复苏细胞系(STR基因鉴定图谱) BS-C02767937S 背景信息：详见相关文献介绍产品包装：复苏：T25培养瓶(1瓶)或冻存：1ml冻存管(2支) 生长特性：贴壁或悬浮，详见说明书部分 1A6 Cell;1A6复苏细胞系(STR基因鉴定图谱) 很多 " - Jeg-3 cell line

Jeg-3 cell line

WebNorfluoxetine increased MMP-9 activity in JEG-3 cells by 2.0% at 0.03 μM and by 43.9% at 3 μM, but decreased MMP-9 activity in HIPEC cells by 63.7% at 3 μM. Sertraline at 0.03 μM increased mRNA level of TIMP-1 in JEG-3 cells by 36% and that of ADAM-10 by 85% and 115% at 0.3 and 3 μM, respectively.

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WebOne of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. ... JEG-3 Product Name: JEG-3. Price: Get Quote: Cat.No: ABC-TC0467. Species: Human. Size/Quantity: 1 vial. Biosafety Level: 1. ... Cell line releases chorionic gonadotrophin and somatomammotrophin and ... Web29 gen 2024 · The JEG-3 choriocarcinoma cell line serves as a model to study EVT cell behavior in the presence of . MPO as primary trophoblasts lack su fficient proliferation in vitro (22).

Web9 mag 2024 · The miR-371-3 cluster (including miR-371, -372, and -373) is expressed in primary trophoblast cells, JEG-3 cells, and their hybrids, but not in immortalized HTR-8/SVneo Mice have homologous ... regulation of Wnt/ β-catenin signaling pathway by miR-424-5p and affects invasion and migration of the trophoblastic cell line HTR-8/SVneo WebHuman choriocarcinoma trophoblastic cells (JEG-3) were used as positive control for the constitutive expression of HLA-G [53]. ... Firstly, we conﬁrmed that 7221.221 B-LCL cell line did not express any HLA antigen (Fig. 1a, b), while 721.221-HLA-G1-transfected cell line (221-G1) constitutively

WebIn this study, we found that the human HLA-G-positive choriocarcinoma cell line JEG-3 upregulates CD4 + CD25 hi CD127 lo T cells, increases the expression of HLA-G + CD4 + and CD8 + T cells, and decreases the expression of ILT2 + on CD4 + T cells in resting PBMCs after six days of co-culture. WebCell line: JEG-3 : DSMZ no.: ACC 463: Species validation: human (Homo sapiens)Cell type: choriocarcinoma : Origin: established from a human gestational choriocarcinoma …

JEG-3 is a hypertriploid, clonally-derived, human cell line with epithelial morphology that was isolated from the Woods strain of the Erwin-Turner tumor. This cell line is tumorigenic, capable of forming malignant tumors consistent with choriocarcionoma. Genes expressed: human chorionic gonadotropin (hCG); human chorionic somatomammotropin ...

Web13 apr 2024 · To verify the function of miR-526b-5p on the trophoblast cells and explore the intrinsic molecular mechanism, transcriptome sequencing was performed on the HTR8 cells overexpressing miR-526b-5p. The miR-526b-5p overexpressing HTR8 cell line was used as the experimental group. The pCD513B-1 empty vector cell line was transfected as the … rainbow gathering 2021WebThe Jeg-3 cell line was used as a positive control. Results: Membrane-bound HLA-G expression levels decreased significantly in both cell lines after transfection with both … rainbow gateway mount isaWebHuman chorionic gonadotrop significantly improved chemotherapy efficacy for the treatment of malignant trophoblastic tumors- Read the full article online rainbow gathering 2022 coloradoWebOne of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. ... JEG-3 Product Name: … rainbow gatheringWeb28 dic 2016 · JEG-3 is a line of choriocarcinoma cells that originated from choriocarcinoma explants. They are phenotypically similar to extravillous trophoblasts (EVTs). JEG-3 cells express human choriogonadotropin; human leukocyte antigen-G, integrin α (ITGA)1, 5 and 6; and MMP2 and MMP9; and have been extensively used as an in vitro model to study … rainbow gathering 2022 locationWeb9 mar 2024 · The JEG-3 cell line is derived from a xenograft of human choriocarcinoma and associated with abnormal placental development and tumor growth ( 5, 19 ). Online RNA-sequencing data showed that RSPO3 is highly expressed in human placental tissues, which are rich in blood vessels ( 1, 2 ). rainbow gathering 2022 mapWeb29 nov 2024 · The BeWo cell line is used to study placental function, since it forms syncytium and secretes hormones after treatment with cAMP or forskolin. In contrast, the … rainbow gathering 2022 colorado facebook