Phis2

WebbSnapGene Viewer lets you see your data for free with the same rich visualization, annotation, and sharing capabilities as the fully enabled SnapGene software. SnapGene … Webb19 sep. 2024 · These sequences were cloned into pHIS2 (Clontech, Palo Alto, CA, USA) upstream of the reporter gene HIS3. Co-transformation of pHIS2 with or without the TCP cis-element sequences and GbTCP4-pGADT7 into Y187 cells was performed as described by Yu et al. (2024) . The pGADT7 vector carries the LEU2 nutritional marker for selection …

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WebbStack Exchange network consists of 181 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, … Webb21 mars 2024 · 苹果腐烂病是苹果生产中最具破坏性的病害。. 在苹果树上添加钾可以有效地控制这种疾病。. 然而，苹果在高钾状态下对苹果腐烂病抗性的潜在机制仍然未知。. 在这里，我们发现高K（HK，9.30 g/kg）苹果组织表现出较高的抗病性。. 当阻断K通道时，阻力受 … ophthalmologist in rice lake wi https://jocatling.com

CmERF5‐CmRAP2.3 transcriptional cascade positively regulates ...

Webb10 juni 2016 · Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To ... WebbVector Map pGADT7-Rec Vector (Not sold separately) Sold as a part of Cat. Nos. 630490 & 630491 (102016) Page 2 of 4 . Figure 2. pGADT7-Rec Vector cloning site. WebbDownload scientific diagram Self-activation detection of pHis2-element recombinant vectors. Transcriptional autoactivation of Y187 recombinant yeast for element No.1 and … ophthalmologist in roanoke va

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Category:Addgene: Vector Database - pHIS-2

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Phis2

Transcription Factor-Centered Yeast One-Hybrid Assay

Webb29 feb. 2024 · The empty vector pGADT7-Rec2 and the pHIS2-cis/promoter were co-transformed as the control for mating experiments. DNA–protein interactions were …

Phis2

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Webb酵母单杂. 使用pHIS2载体，将启动子序列构建在HIS3报告基因前面的多克隆位点上，对应的转录因子构建在AD载体上即可。. 使用Y187菌株，它相对于AH109菌株在基因组上少 … Webb27 mars 2015 · To suppress potential background activity, the medium was complemented with 50 or 100 m m 3-amino-1,2,4-triazole (3-AT) and yeast strains harbouring recombinant pHis2.1 were plated on this medium in different dilutions. In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth.

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Webb27 mars 2024 · The Y187 yeast strains containing recombined pHIS2 vectors were first cultivated in −T−H screening medium with different concentrations of 3-amino-1,2,4-triazole (3-AT), which was used to suppress background histidine leakiness of the pHIS2 vector. The concentration of 3-AT that prevented yeast growth in −T−H screening … WebbDescription. District Health Information Software 2 Tracker (DHIS2 Tracker) is an open-source, web-based application that supports data collection, and analysis of …

Webb10 aug. 2014 · The interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches …

Webb29 okt. 2024 · To test whether CmMYB9a directly binds to the promoters of these four structural genes, yeast one-hybrid assays were performed with co-transformation of pGADT7 (AD)-CmMYB9a constructs and pHIS2 vectors containing the promoters. Co-transformation of the AD vector and these pHIS2 constructs served as negative controls. portfolio thank you letterWebb1 mars 2015 · The cotransformation of p53HIS2 (three tandem copies of the p53 DNA element in pHIS2 which is recognized by p53) and pGADT7-p53 (encodes murine p53 as a fusion with the GAL4 AD) was used as positive control. The cotransformation of p53HIS2 and pGADT7-ATDOF5.8 was used as negative controls. portfolio text imagesWebbpHIS2 Vector Information Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3705-5 2 Version No. PR32049 Notice to Purchaser This product is intended to be … ophthalmologist in rockwallWebb24 jan. 2024 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies … portfolio that never losesWebbphis-docs-community Overview. This repository contains OpenSILEX PHIS users documentation. Background. PHIS (Phenotyping Hybrid Information System) is … ophthalmologist in radcliff kyhttp://www.honorgene.com/product_list/carrier_library/123112.html ophthalmologist in rolla moWebb12 apr. 2024 · 新-酵母单杂交筛选与启动子phis2-a互作蛋白结题报告; 配套k122024届高考化学一轮复习 选考 有机化学基础 第1节 有机化合物的组; 职业技术学院智慧校园系统需求规划知识讲解; 幼儿园小班教案《美丽花园》含反思; 档案数字化服务外包规范; 环境保护行政复议 … ophthalmologist in sanford fl